Coding

Part:BBa_K2016004:Design

Designed by: Magdalena Dabrowska   Group: iGEM16_Sheffield   (2016-10-07)


TdHr - iron-responsive, oxygen-responsive hemerythrin protein coding gene from Themiste dyscritum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 338
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genetics sequence has been codon optimised for E. coli, gene cloned between XbaI and SpeI sites.


Source

Published sequence of oxygenated form - 1HMO

References